2W1R
Crystal Structure of the C-terminal Domain of B. subtilis SpoVT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.930, 63.930, 77.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.22332 |
| Rwork | 0.222 |
| R-free | 0.24217 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.990 |
| Data reduction software | MOSFLM |
| Data scaling software | XSCALE |
| Phasing software | RESOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.190 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.080 | 0.270 |
| Number of reflections | 19148 | |
| <I/σ(I)> | 14.3 | 4.7 |
| Completeness [%] | 97.8 | 91.3 |
| Redundancy | 11.5 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 200 MM NACL, 100 MM HEPES PH 7.5, 25% PEG 3350 |
