2W0Z
Grb2 SH3C (3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-09-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.150, 28.705, 45.114 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.130 - 1.700 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vdf |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.334 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.240 |
| Number of reflections | 7072 | |
| <I/σ(I)> | 38.7 | 6.3 |
| Completeness [%] | 98.8 | 84.3 |
| Redundancy | 6.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.7 M TRISODIUM CITRATE, 0.1 M TRIS PH 8.5 |
