2W0R
Crystal structure of the mutated N263D YscU C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-12 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 66.473, 66.473, 68.113 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.550 |
R-factor | 0.202 |
Rwork | 0.201 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v5g |
RMSD bond length | 0.019 |
RMSD bond angle | 1.698 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.630 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.050 | 0.150 |
Number of reflections | 21871 | |
<I/σ(I)> | 8.4 | 4.8 |
Completeness [%] | 96.7 | 99.9 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | HANGING DROP METHOD USING 3 UL OF A 8 MG/ML PROTEIN SOLUTION MIXED WITH 3 UL RESERVOIR BUFFER (1.6 M (NH4)2SO4, 0.2 M NACL, 0.1 M HEPES PH 7.5) IN THE DROPLET. |