2W00
Crystal structure of the HsdR subunit of the EcoR124I restriction enzyme in complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-20 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 123.910, 129.922, 161.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.600 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.26500 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.014 |
RMSD bond angle | 1.418 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.130 | 0.500 |
Number of reflections | 80316 | |
<I/σ(I)> | 17.9 | 5.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.4 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1 UL OF PROTEIN IN 20 MM PHOSPHATE PH 7.5, 100 MM KCL, 5 MM ATP WAS MIXED WITH 2 UL OF RESERVOIR, CONTAINING 0.2 M LI2SO4, 8 % PEG 20K, 8 % PEG 550 MME, 1.5 MM DTT, 277 K, SITTING DROP |