2VZY
Crystal structure of Rv0802c from Mycobacterium tuberculosis in an unliganded form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-20 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.705, 111.458, 135.238 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.070 - 2.000 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.20500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.546 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (RESOLVE) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.180 |
| Number of reflections | 69624 | |
| <I/σ(I)> | 22.3 | 4.8 |
| Completeness [%] | 95.4 | 79.4 |
| Redundancy | 5.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.25 | PROTEIN AT 7MG/ML IN 10 MM TEA PH 7.8, 100 MM AMMONIUM SULFATE, 3% GLYCEROL, 1 MM EDTA, 2 MM DTT CRYSTALLIZED IN 150 MM NACITRATE PH 5.25 |
