2VZG
Crystal structure of the C-terminal calponin homology domain of alpha- parvin in complex with paxillin LD2 motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-02 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 74.008, 94.422, 41.841 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.220 - 1.800 |
R-factor | 0.187 |
Rwork | 0.186 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vzc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.225 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.710 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.350 |
Number of reflections | 13920 | |
<I/σ(I)> | 13.2 | 3.2 |
Completeness [%] | 99.6 | 100 |
Redundancy | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 286 | 40% (W/V) PEG200, 0.1 M CITRATE PH 4.5 |