2VZA
Type IV secretion system effector protein BepA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-16 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 229.902, 229.902, 308.994 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 3.050 |
R-factor | 0.246 |
Rwork | 0.244 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vy3 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.017 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.620 | 3.210 |
High resolution limit [Å] | 2.950 | 3.050 |
Rmerge | 0.110 | 0.450 |
Number of reflections | 55048 | |
<I/σ(I)> | 5.34 | 1.61 |
Completeness [%] | 92.6 | 75.4 |
Redundancy | 2.91 | 1.84 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.25 | 30 MG/ML PROTEIN IN 20 MM TRIS_HCL (PH 8.0), 500 MM NACL AND 2 MM BETA-MERCAPTOETHANOL MIXED WITH 1.5 M AMMONIUM SULFATE, 10 MM TRIS-HCL (PH 8.25) AND 0.1 M LITHIUM SULFATE |