2VZ8
Crystal Structure of Mammalian Fatty Acid Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 10 |
| Detector technology | CCD |
| Collection date | 2006-12-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.320, 244.700, 135.250 |
| Unit cell angles | 90.00, 101.65, 90.00 |
Refinement procedure
| Resolution | 29.496 - 3.219 |
| R-factor | 0.2196 |
| Rwork | 0.217 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cf2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.231 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.170 | 0.990 |
| Number of reflections | 96975 | |
| <I/σ(I)> | 9.9 | 1.4 |
| Completeness [%] | 98.5 | 93.2 |
| Redundancy | 7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
