2VZ3

bleached galactose oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	96.480, 88.090, 84.940
Unit cell angles	90.00, 116.67, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.1685
Rwork	0.169
R-free	0.20250
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.990
High resolution limit [Å]	1.900	1.900
Rmerge	0.040	0.126
Number of reflections	47220
<I/σ(I)>	11.7
Completeness [%]	94.3	88.8
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6		20% PEG 8000, 100MM MES PH 6.0, 200MM CALCIUM ACETATE