2VZ1
Premat-galactose oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.481, 88.088, 84.935 |
| Unit cell angles | 90.00, 116.67, 90.00 |
Refinement procedure
| Resolution | 28.750 - 1.910 |
| R-factor | 0.17711 |
| Rwork | 0.177 |
| R-free | 0.22272 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t2x |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 49382 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 98.7 | 93.8 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 20% PEG 8000, 100MM MES PH 6.0, 200MM CALCIUM ACETATE |
