2VYV

Structure of E.Coli GAPDH Rat Sperm GAPDH heterotetramer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-16
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	68.445, 103.562, 177.832
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.620 - 2.380
R-factor	0.184
Rwork	0.180
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yvn
RMSD bond length	0.010
RMSD bond angle	1.271
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.600	2.430
High resolution limit [Å]	2.400	2.380
Rmerge	0.070
Number of reflections	51658
<I/σ(I)>	10
Completeness [%]	94.5	78.1
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		0.2M NA FORMATE, 20% PEG 3350, 0.1M HEPES PH 7.5