2VYO
Chitin deacetylase family member from Encephalitozoon cuniculi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 190 |
| Detector technology | CCD |
| Collection date | 2005-12-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.975, 81.916, 39.201 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c1g |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 33981 | |
| <I/σ(I)> | 19.5 | 2.26 |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 10% PEG 8000, 100 MM IMIDAZOLE 200 MM CALCIUM ACETATE 10MM ZNCL 20C, pH 8.0 |
