2VYC
Crystal Structure of Acid Induced Arginine Decarboxylase from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2006-12-07 |
Detector | ADSC CCD |
Spacegroup name | P 64 |
Unit cell lengths | 197.651, 197.651, 450.322 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.179 |
Rwork | 0.177 |
R-free | 0.22900 |
Structure solution method | MIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.013 |
RMSD bond angle | 1.345 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.150 | 0.780 |
Number of reflections | 385411 | |
<I/σ(I)> | 10.7 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 100 MM MES PH 6.5, 13% PEG 8000 AND 400 MM SODIUM ACETATE | ||
1 | 6.5 | 100 MM MES PH 6.5, 13% PEG 8000 AND 400 MM SODIUM ACETATE | ||
1 | 6.5 | 100 MM MES PH 6.5, 13% PEG 8000 AND 400 MM SODIUM ACETATE |