2VY8
The 627-domain from influenza A virus polymerase PB2 subunit with Glu- 627
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-10 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 48.500, 45.700, 60.840 |
Unit cell angles | 90.00, 106.24, 90.00 |
Refinement procedure
Resolution | 29.210 - 1.200 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 627-DOMAIN WITH K627 FROM PDB ENTRY 2VY7 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.537 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.200 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.100 | 0.330 |
Number of reflections | 42201 | |
<I/σ(I)> | 5.07 | 2.5 |
Completeness [%] | 92.9 | 85.9 |
Redundancy | 2.84 | 1.39 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1 MICROLITRE OF 2.4 MG/ML PROTEIN SOLUTION IN 10 MM TRIS-HCL PH 7.0 AND 200 MM NACL WITH 1 MICROLITRE OF 100 MM CITRIC ACID PH 4.0-7.0 AND 1.4-1.6 M AMMONIUM SULFATE SOLUTION |