2VY7
The 627-domain from influenza A virus polymerase PB2 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-19 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 48.390, 45.020, 60.000 |
| Unit cell angles | 90.00, 105.45, 90.00 |
Refinement procedure
| Resolution | 57.830 - 1.530 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.18500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.573 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.040 | 0.170 |
| Number of reflections | 36025 | |
| <I/σ(I)> | 20.2 | 5.3 |
| Completeness [%] | 97.6 | 77.3 |
| Redundancy | 3.67 | 2.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1 MICROLITRE OF 2.4 MG/ML PROTEIN SOLUTION IN 10 MM TRIS-HCL PH 7.0 AND 200 MM NACL WITH 1 MICROLITRE OF 100 MM CITRIC ACID PH 4.0-7.0 AND 1.4-1.6 M AMMONIUM SULFATE SOLUTION |
