2VY0
The X-ray structure of endo-beta-1,3-glucanase from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.363, 84.762, 69.232 |
| Unit cell angles | 90.00, 104.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.160 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hyk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.349 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.080 | 0.210 |
| Number of reflections | 25211 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES [N-(2-HYDROXYETHYL)PIPERAZINE-N-2-ETHANESULFONIC ACID]PH 7.5-8.0 MPD (2-METHYL-2,4-PENTANEDIOL) 60%-70% |
