2VXP

The fourth FAS1 domain structure of human Bigh3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 65
Unit cell lengths	62.934, 62.934, 143.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.27
Rwork	0.270
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.970
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.110	0.610
Number of reflections	21986
<I/σ(I)>	35.38	5.04
Completeness [%]	100.0	100
Redundancy	16.9	15.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1