2VXB
Structure of the Crb2-BRCT2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-20 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.887, 73.733, 66.772 |
Unit cell angles | 90.00, 110.98, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.300 |
Rwork | 0.189 |
R-free | 0.24900 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.620 |
Number of reflections | 29876 | |
<I/σ(I)> | 11.3 | 1.6 |
Completeness [%] | 99.0 | 93.8 |
Redundancy | 3.9 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | CRB2-BRCT2 (7MG/ML IN 0.05M TRIS-HCL PH7.0, 1M NACL,5MM DTT) WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 20C. PLATECLUSTERS GREW FROM 2UL PROTEIN, 2UL WELL SOLUTION (0.1M NA-CACODYLATE PH6.5, 7.0% PEG8000 AND 0.2M CA-ACETATE) AND 0.5UL 0.1M PR(III) ACETATE. |