2VX7

CELLVIBRIO JAPONICUS MANNANASE CJMAN26C MANNOBIOSE-BOUND FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-13
Detector	ADSC CCD
Spacegroup name	P 61 2 2
Unit cell lengths	84.334, 84.334, 243.295
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.940 - 1.800
R-factor	0.15137
Rwork	0.150
R-free	0.17648
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j9y
RMSD bond length	0.010
RMSD bond angle	1.223
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.4.0076)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.090	0.410
Number of reflections	48533
<I/σ(I)>	16.2	2.9
Completeness [%]	99.9	100
Redundancy	9.5	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1