2VX2
Crystal structure of human enoyl Coenzyme A hydratase domain- containing protein 3 (ECHDC3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-10 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 235.756, 135.296, 83.871 |
Unit cell angles | 90.00, 98.84, 90.00 |
Refinement procedure
Resolution | 117.040 - 2.300 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uiy |
RMSD bond length | 0.020 |
RMSD bond angle | 1.633 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 117.000 | 2.460 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.080 | 0.520 |
Number of reflections | 118152 | |
<I/σ(I)> | 9.7 | 2.3 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.20M NA(FORM); 0.1M BTPROP PH 8.5, 20.0% PEG350, 10.0% ETGLY |