2VWR
Crystal structure of the second pdz domain of numb-binding protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-10 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.773, 38.643, 38.186 |
| Unit cell angles | 90.00, 117.22, 90.00 |
Refinement procedure
| Resolution | 33.960 - 1.300 |
| R-factor | 0.136 |
| Rwork | 0.134 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2QG1 AND 2HE2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.313 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.960 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.060 | 0.240 |
| Number of reflections | 19996 | |
| <I/σ(I)> | 16 | 5.2 |
| Completeness [%] | 98.0 | 88.9 |
| Redundancy | 5.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 40% PEG 300, 0.1M CITRATE/PO4 PH 4.2 |
