2VWP
Haloferax mediterranei glucose dehydrogenase in complex with NADPH and Zn.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-26 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 60.569, 107.725, 153.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 2.010 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b5v |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.287 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.060 | 0.350 |
| Number of reflections | 32173 | |
| <I/σ(I)> | 30.1 | 3.7 |
| Completeness [%] | 94.5 | 82.5 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 70% 2-METHYL-2,4,-PENTANEDIOL, 1MM NADPH, 1MM ZNCL2, 100MM HEPES PH7.5 |
