2VWA
Crystal structure of a sporozoite protein essential for liver stage development of malaria parasite
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.544, 38.274, 145.696 |
| Unit cell angles | 90.00, 90.25, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.476 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.290 |
| Number of reflections | 32131 | |
| <I/σ(I)> | 29.65 | 8.1 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 9.9 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN WAS CRYSTALLIZED FROM 0.1 M MES PH 6.5, 12% PEG 20,000 AND 0.01 M DTT |
