2VVP
Crystal structure of Mycobacterium tuberculosis ribose-5-phosphate isomerase B in complex with its substrates ribose 5-phosphate and ribulose 5-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2005-05-05 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.832, 103.058, 69.728 |
Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
Resolution | 34.710 - 1.650 |
R-factor | 0.168 |
Rwork | 0.166 |
R-free | 0.18700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1usl |
RMSD bond length | 0.011 |
RMSD bond angle | 1.437 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC (5) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.070 | 0.290 |
Number of reflections | 115358 | |
<I/σ(I)> | 13.3 | 3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% PEG 3000, 0.1 M TRIS PH 7, 0.2 M CA ACETATE |