2VVI
IrisFP fluorescent protein in its green form, trans conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-16 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.310, 96.380, 140.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.570 - 2.000 |
R-factor | 0.1961 |
Rwork | 0.193 |
R-free | 0.25138 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zux |
RMSD bond length | 0.009 |
RMSD bond angle | 1.365 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.600 | 2.200 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.100 | 0.470 |
Number of reflections | 73301 | |
<I/σ(I)> | 10.1 | 2.8 |
Completeness [%] | 97.3 | 98.6 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.4 | 293 | CRYSTALLIZATION CONDITIONS: AMMONIUM SULFATE, BICINE, PH 8.4, VAPOR DIFFUSION, TEMPERATURE 293K |