2VVE
Crystal structure of the stem and receptor binding domain of the spike protein P1 from bacteriophage PM2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-30 |
Detector | MARRESEARCH |
Spacegroup name | P 4 |
Unit cell lengths | 107.894, 107.894, 44.171 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.000 - 1.770 |
R-factor | 0.176 |
Rwork | 0.173 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vvd |
RMSD bond length | 0.009 |
RMSD bond angle | 1.097 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.000 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.080 | 0.870 |
Number of reflections | 47548 | |
<I/σ(I)> | 30.2 | 1.8 |
Completeness [%] | 94.9 | 71.8 |
Redundancy | 11.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 7.3MG/ML (IN 150MM NACL, 20MM TRIS PH 7.5, 10MM CACL2),20%(W/V) PEG6K,100MM TRIS PH 8, 200MM LICL |