2VVB
Human carbonic anhydrase II in complex with bicarbonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.667, 41.653, 72.910 |
| Unit cell angles | 90.00, 104.79, 90.00 |
Refinement procedure
| Resolution | 70.710 - 1.660 |
| R-factor | 0.171 |
| Rwork | 0.168 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cba |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.587 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.990 | 1.730 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.070 | 0.280 |
| Number of reflections | 29567 | |
| Completeness [%] | 87.7 | 78.2 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.4 M (NH4)2SO4. 0.1 M TRIS PH 8.5 |
