2VV9
CDK2 in complex with an imidazole piperazine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-11 |
| Detector | ADSC QUANTUM-4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.252, 71.153, 71.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.520 - 1.900 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE CDK2 STRUCTURE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.501 |
| Data reduction software | MOSFLM |
| Data scaling software | TRUNCATE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.520 | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.320 |
| Number of reflections | 21456 | |
| <I/σ(I)> | 16.05 | 1.8 |
| Completeness [%] | 97.3 | 88.3 |
| Redundancy | 2.67 | 2.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 8% PEG 3350, 0.1M HEPES PH 7.5, 0.05M AMMONIUM ACETATE |
