2VV8
Co-bound structure of bjFixLH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 48.923, 50.296, 58.993 |
Unit cell angles | 73.37, 70.26, 72.13 |
Refinement procedure
Resolution | 46.880 - 1.610 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xj3 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.643 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.670 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.040 | 0.320 |
Number of reflections | 57814 | |
<I/σ(I)> | 29 | 4 |
Completeness [%] | 90.7 | 93.7 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | NACL, PEI, CAPSO, PH 9.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |