2VUU

Crystal structure of NADP-bound NmrA-AreA zinc finger complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	H 3
Unit cell lengths	231.730, 231.730, 223.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.860 - 2.800
R-factor	0.232
Rwork	0.232
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1K6J AND 4GAT
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.140	0.860
Number of reflections	110086
<I/σ(I)>	11.4	1.4
Completeness [%]	100.0	100
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.4		0.2M LI2SO4, 0.1M BIS-TRIS PH 6.4, 15% - 17% PEG3350