2VUL
Thermostable mutant of ENVIRONMENTALLY ISOLATED GH11 XYLANASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-12 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.669, 33.594, 83.142 |
| Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
| Resolution | 31.780 - 1.900 |
| R-factor | 0.153 |
| Rwork | 0.150 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vuj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.421 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 12430 | |
| <I/σ(I)> | 20.8 | 2 |
| Completeness [%] | 93.0 | 61.1 |
| Redundancy | 7.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.1 | 55% (W/V) PEG 400, 0.15 M LITHIUM SULPHATE, 0.1 M TRI-SODIUM ACETATE (PH 5.1) |
