2VUE

Human serum albumin complexed with 4Z,15E-bilirubin-IX-alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	298
Detector technology	CCD
Collection date	2003-02-15
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	55.090, 55.540, 119.830
Unit cell angles	81.22, 90.70, 65.42

Refinement procedure

Resolution	35.070 - 2.420
R-factor	0.218
Rwork	0.218
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bxa
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.070	2.570
High resolution limit [Å]	2.420	2.420
Rmerge	0.040	0.310
Number of reflections	45968
<I/σ(I)>	9.8	2.2
Completeness [%]	94.9	85.8
Redundancy	1.9	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		SEE PAPER, pH 7