2VU1
Biosynthetic thiolase from Z. ramigera. Complex of with O-pantheteine- 11-pivalate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.298, 78.737, 148.339 |
| Unit cell angles | 90.00, 92.93, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.510 |
| R-factor | 0.21574 |
| Rwork | 0.215 |
| R-free | 0.24726 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.709 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.3.0028) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.090 | 0.550 |
| Number of reflections | 284637 | |
| <I/σ(I)> | 8.7 | 2.1 |
| Completeness [%] | 93.8 | 70.1 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | pH 5 |
