2VU0
Biosynthetic thiolase from Z. ramigera. Complex of the oxidised enzyme with coenzyme A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.402, 79.056, 148.802 |
| Unit cell angles | 90.00, 92.66, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.870 |
| R-factor | 0.19549 |
| Rwork | 0.194 |
| R-free | 0.22947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.603 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.950 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.070 | 0.190 |
| Number of reflections | 161643 | |
| <I/σ(I)> | 12.1 | 4.4 |
| Completeness [%] | 97.3 | 86.3 |
| Redundancy | 3.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | pH 5 |
