2VTU
crystal structure of bacteriophage MS2 covalent coat protein dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-19 |
| Detector | ADSC CCD |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 220.438, 220.438, 220.438 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 3.500 |
| R-factor | 0.2723 |
| Rwork | 0.272 |
| R-free | 0.31020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.054 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.150 | 0.360 |
| Number of reflections | 6079 | |
| <I/σ(I)> | 17.3 | 6.7 |
| Completeness [%] | 97.8 | 99.2 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.32M NA2HPO4, 0.08M NAH2PO4 AND 5% PEG 8000, pH 7.5 |
