2VTC
The structure of a glycoside hydrolase family 61 member, Cel61B from the Hypocrea jecorina.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 101.445, 101.445, 102.697 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.351 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.370 |
| Number of reflections | 9041 | |
| <I/σ(I)> | 6.3 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 15-20 % PEG 2000, 0.1 M TRIS PH 8.4 AND 10MM NICKEL CHLORIDE. |
