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2VT8

Structure of a conserved dimerisation domain within Fbox7 and PI31

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2004-01-06
DetectorMARRESEARCH
Spacegroup nameC 1 2 1
Unit cell lengths109.498, 45.212, 67.279
Unit cell angles90.00, 111.54, 90.00
Refinement procedure
Resolution28.090 - 2.600
R-factor0.206
Rwork0.203
R-free0.25600
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)PART-REFINED MODEL OF A L7M SEMET DERIVATIVE OBTAINED BY SAD PHASING
RMSD bond length0.023
RMSD bond angle2.155
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]28.1002.740
High resolution limit [Å]2.6002.600
Rmerge0.0800.390
Number of reflections9607
<I/σ(I)>20.14.1
Completeness [%]99.697.9
Redundancy7.16.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1820% PEG 3350, 0.1 M AMMONIUM IODIDE, pH 8

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