2VT2
Structure and functional properties of the Bacillus subtilis transcriptional repressor Rex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-16 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.290, 53.740, 86.890 |
| Unit cell angles | 90.00, 125.55, 90.00 |
Refinement procedure
| Resolution | 28.610 - 2.300 |
| R-factor | 0.221 |
| Rwork | 0.217 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vt3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.643 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.010 | 0.040 |
| Number of reflections | 21294 | |
| <I/σ(I)> | 19.1 | 5 |
| Completeness [%] | 97.9 | 98.5 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 20% PEG6000, 0.2M NH4CL, 0.1M HEPES PH 7.1 |
