2VSV
Crystal structure of the PDZ domain of human rhophilin-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-09 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.250, 39.890, 73.480 |
| Unit cell angles | 90.00, 115.78, 90.00 |
Refinement procedure
| Resolution | 66.230 - 1.820 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2F5Y 2ocs 2v90 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.376 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 1.920 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.100 | 0.330 |
| Number of reflections | 18177 | |
| <I/σ(I)> | 8.8 | 3.2 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M SODIUM ACETATE, 20% PEG3350; 10% ETHYLENE GLYCOL, pH 7.5 |
