2VSP
Crystal structure of the fourth PDZ domain of PDZ domain-containing protein 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-10 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 99.327, 99.327, 97.114 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.670 - 2.410 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.024 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.390 | 2.850 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.100 | 0.520 |
| Number of reflections | 14236 | |
| <I/σ(I)> | 13.7 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50% PEG 300; 0.20M NACL; 0.1M NA/K-PO4 PH 6.2 |
