2VSK
Hendra virus attachment glycoprotein in complex with human cell surface receptor ephrinB2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 77.2 |
| Detector technology | CCD |
| Collection date | 2006-05-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.250, 106.170, 196.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.3 |
| Rwork | 0.300 |
| R-free | 0.35000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vsm NIV-G-EFNB2 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.518 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.210 | 0.830 |
| Number of reflections | 17229 | |
| <I/σ(I)> | 6.1 | 2 |
| Completeness [%] | 93.9 | 82.7 |
| Redundancy | 7.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25% PEG 3350 0.1 M BIS-TRIS PH 5.5 |
