2VSI
Synthesis of CDP-activated ribitol for teichoic acid precursors in Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.940, 92.380, 143.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.080 - 2.750 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vpa |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.193 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.860 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.090 | 0.520 |
| Number of reflections | 14243 | |
| <I/σ(I)> | 14.7 | 2.2 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.75 | 100 MM HEPES PH 6.75 35 % V/W PEG 300 200 MM CACL2 |
