2VSA
Structure and mode of action of a mosquitocidal holotoxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-26 |
Detector | MARREASEARCH |
Spacegroup name | P 3 |
Unit cell lengths | 129.974, 129.974, 68.816 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.890 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cb4 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.390 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.080 | 0.330 |
Number of reflections | 29077 | |
<I/σ(I)> | 11.8 | 4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 3% (W/V) PEG-6000 0.1 M TRIS, PH 8.5 0.1 M KCL |