2VSA
Structure and mode of action of a mosquitocidal holotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-26 |
| Detector | MARREASEARCH |
| Spacegroup name | P 3 |
| Unit cell lengths | 129.974, 129.974, 68.816 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.890 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cb4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.390 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.330 |
| Number of reflections | 29077 | |
| <I/σ(I)> | 11.8 | 4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 3% (W/V) PEG-6000 0.1 M TRIS, PH 8.5 0.1 M KCL |
