2VS7
The crystal structure of I-DmoI in complex with DNA and Ca
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9, 1.5414 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.988, 70.587, 106.848 |
| Unit cell angles | 90.00, 119.75, 90.00 |
Refinement procedure
| Resolution | 25.760 - 2.050 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.450 |
| Number of reflections | 87351 | |
| <I/σ(I)> | 14.9 | 1 |
| Completeness [%] | 95.4 | 72.5 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6% PEG 4000, 0.07M SODIUM ACETATE, 30% GLYCEROL |
