2VS4
THE BINDING OF UDP-GALACTOSE BY AN ACTIVE SITE MUTANT OF alpha-1,3 GALACTOSYLTRANSFERASE (alpha3GT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-03 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.251, 94.630, 94.690 |
Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
Resolution | 32.500 - 1.770 |
R-factor | 0.263 |
Rwork | 0.263 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4v |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.070 | 0.230 |
Number of reflections | 76955 | |
<I/σ(I)> | 20.4 | 3.6 |
Completeness [%] | 92.3 | 87.5 |
Redundancy | 5.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | PROTEIN: 10 MM UDP-GAL, 10 MM MNCL2, 10 MM N-ACETYLLACTOSAMINE, MOTHER LIQUOR: 5-10 % PEG 6000, 0.1 M TRIS-HCL PH 8.0, 5-14 % MPD |