2VS0
Structural analysis of homodimeric staphylococcal aureus virulence factor EsxA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.773, 39.069, 43.029 |
| Unit cell angles | 67.41, 84.51, 88.76 |
Refinement procedure
| Resolution | 39.560 - 1.400 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.21800 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.650 |
| Number of reflections | 28979 | |
| <I/σ(I)> | 16.1 | 2 |
| Completeness [%] | 96.0 | 92 |
| Redundancy | 3.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1M MES PH 5.5, 80MM ZINC ACETATE, 8% PEG 8000 |
