2VR9

Drosophila Robo IG1-2 (tetragonal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-05
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	115.398, 115.398, 144.714
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.200
R-factor	0.2374
Rwork	0.237
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iep
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.110	0.450
Number of reflections	15618
<I/σ(I)>	11.9	3
Completeness [%]	94.6	95
Redundancy	4.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1