2VR5
Crystal structure of Trex from Sulfolobus Solfataricus in complex with acarbose intermediate and glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 97 |
Detector technology | CCD |
Collection date | 2007-11-15 |
Detector | ADSC CCD |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 204.814, 204.814, 89.558 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.850 - 2.800 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.26600 |
Structure solution method | MIR |
Starting model (for MR) | 2vnc |
RMSD bond length | 0.006 |
RMSD bond angle | 0.600 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.090 | 0.270 |
Number of reflections | 51526 | |
<I/σ(I)> | 15.4 | 3.7 |
Completeness [%] | 96.6 | 93 |
Redundancy | 6.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | PEG 3K 8%, 0.2M LITHIUM SULFATE, 0.1M PH 8.0 IMIDAZOLE BUFFER |