2VR2
Human Dihydropyrimidinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 89.650, 89.650, 219.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.950 - 2.800 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ftw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 0.660 |
| Number of reflections | 13425 | |
| <I/σ(I)> | 26.63 | 6.01 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 17.23 | 17.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH 7.5, 70% MPD |
