2VR1
Crystal structure of Biotin carboxylase from E. coli in complex with ATP analog, ADPCF2P.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Collection date | 2007-05-07 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.129, 106.784, 121.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dv2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.111 |
| Data reduction software | SCALEPACK |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.100 | 0.540 |
| Number of reflections | 32843 | |
| <I/σ(I)> | 17.83 | 3.3 |
| Completeness [%] | 96.0 | 96.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M KCL, 3-8% PEG 8000 |
